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style=\"text-align: center\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/www.qipaizn.cn\/wp-content\/uploads\/2023\/04\/5.jpg\" alt=\"\u6c42\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u8bb2\u4e49\" width=\"600\" height=\"425\" \/><\/p>\n<p style=\"color: #444242;width: 100%;font-size: 60%;background: #F1F1F1;padding: 0px 0px\"><strong>\u4e09\u3001\u4e0d\u9508\u94a2\u53cd\u5e94\u91dc\u600e\u4e48\u5206\u7c7b\u7684\uff0c\u53ef\u4ee5\u7ed9\u8be6\u7ec6\u4ecb\u7ecd\u4e00\u4e0b\u5417\uff1f\u6700\u597d\u662f\u751f\u4ea7\u53cd\u5e94\u91dc\u7684\u4f01\u4e1a\u7ed9\u4e2a\u8be6\u7ec6\u4ecb\u7ecd\u3002<\/strong><\/span><\/p>\n<p>\u53c2\u8003\u8d44\u6599\uff1a<\/p>\n<p style=\"color: #444242;width: 100%;font-size: 60%;background: #F1F1F1;padding: 0px 0px\"><strong>\u56db\u3001\u6c42\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u8bb2\u4e49<\/strong><\/span><\/p>\n<p>\u3010\u5b9e\u9a8c\u76ee\u7684\u3011<br \/>\uff081\uff09\u4e86\u89e3\u5b9e\u9a8c\u7684\u5e38\u89c4\u4eea\u5668\u3001\u8bbe\u5907\u3001\u8017\u6750\u3002<br \/>\uff082\uff09\u638c\u63e1\u672c\u5b9e\u9a8c\u6240\u7528\u4eea\u5668\u7684\u529f\u80fd\u548c\u4f7f\u7528\u65b9\u6cd5\u3002<br \/>\u3010\u5b9e\u9a8c\u5185\u5bb9\u3011<br \/>\u4e00\u3001\u9a8c\u5ba4\u7684\u5e38\u89c4\u4eea\u5668\u3001\u8bbe\u5907<br \/>(\u4e00) \u6e29\u5ea6\u63a7\u5236\u7cfb\u7edf\uff1a<br \/>1. \u51b0\u7bb1: \u6839\u636e\u836f\u54c1\u3001\u8bd5\u5242\u53ca\u591a\u79cd\u751f\u7269\u5236\u5242\u4fdd\u5b58\u7684\u9700\u8981\uff0c\u5fc5\u987b\u5177\u5907\u4e0d\u540c\u63a7\u6e29\u7ea7\u522b\u7684\u51b0\u7bb1\uff0c\u6700\u5e38\u4f7f\u7528\u7684\u6709\uff1a4\u2103\u3001-20\u2103\u3001-80\u2103\u51b0\u7bb1\u3002<br \/>4\u2103\u9002\u5408\u50a8\u5b58\u67d0\u4e9b\u6eb6\u6db2\u3001\u8bd5\u5242\u3001\u836f\u54c1\u7b49\u3002<br \/>-20\u2103\u9002\u7528\u4e8e\u67d0\u4e9b\u8bd5\u5242\u3001\u836f\u54c1\u3001\u9176\u3001\u8840\u6e05\u3001\u914d\u597d\u7684\u6297\u751f\u7d20\u548cDNA\u3001\u86cb\u767d\u8d28\u6837\u54c1\u7b49\u3002<br \/>-80\u2103\u9002\u5408\u67d0\u4e9b\u957f\u671f\u4f4e\u6e29\u4fdd\u5b58\u7684\u6837\u54c1\u3001\u7eaf\u5316\u7684\u6837\u54c1\u3001\u7279\u6b8a\u7684\u4f4e\u6e29\u5904\u7406\u6d88\u5316\u6db2\u7b49\u7684\u4fdd\u5b58\u3002<br \/>0-10\u2103\u7684\u51b7\u67dc\u9002\u5408\u4f4e\u6e29\u6761\u4ef6\u4e0b\u7684\u7535\u6cf3\u3001\u5c42\u6790\u3001\u900f\u6790\u7b49\u5b9e\u9a8c\u3002<br \/>2\uff0e\u6db2\u6c2e\u7f50\uff1a\u6709\u4e9b\u5b9e\u9a8c\u6750\u6599\u3001\u67d0\u4e9b\u5668\u5b98\u7ec4\u7ec7\u3001\u7ec6\u80de\u682a\u3001\u83cc\u682a\u53ca\u7eaf\u5316\u7684\u6837\u54c1\u7b49\uff0c\u8981\u6c42\u901f\u51bb\u548c\u957f\u671f\u4fdd\u5b58\u5728\u8d85\u4f4e\u6e29\u73af\u5883\u4e0b\uff0c\u5c31\u9700\u8981\u4e00\u4e2a\u6db2\u6c2e\u7f50\uff08-196\u2103\uff09\u5177\u6709\u7ecf\u6d4e\u3001\u7701\u529b\u548c\u8f83\u597d\u5730\u4fdd\u6301\u7ec6\u80de\u751f\u7269\u5b66\u7279\u6027\u7684\u4f18\u70b9\u3002<br \/>3\uff0e\u57f9\u517b\u7bb1\uff1a37\u2103\u6052\u6e29\u7bb1\u7528\u4e8e\u7ec6\u83cc\u7684\u56fa\u4f53\u57f9\u517b\u548c\u7ec6\u80de\u57f9\u517b\u3002<br \/>CO2\u57f9\u517b\u7bb1\u9002\u7528\u4e8e\u57f9\u517b\u5404\u79cd\u7ec6\u80de\uff0c\u53ef\u6052\u5b9a\u5730\u63d0\u4f9b\u4e00\u5b9a\u91cf\u7684CO2\uff08\u901a\u5e385%\uff09\uff0c\u7528\u6765\u7ef4\u6301\u57f9\u517b\u6db2\u7684\u9178\u5ea6\uff08pH\u503c\uff09\u3002<br \/>37\u2103\u6052\u6e29\u7a7a\u6c14\u6447\u5e8a\u53ef\u8fdb\u884c\u6db2\u4f53\u7ec6\u83cc\u7684\u57f9\u517b\u3002<br \/>4. \u6c34\u6d74\u9505\uff1a\u7528\u4e8e\u4fdd\u6e29\u3002<br \/>25-100\u2103\u6c34\u6d74\u6447\u5e8a\u53ef\u7528\u4e8e\u5206\u5b50\u6742\u4ea4\u8bd5\u9a8c\uff0c\u5404\u79cd\u751f\u7269\u5316\u5b66\u9176\u53cd\u5e94\u7b49\u8bd5\u9a8c\u7684\u4fdd\u6e29\u3002<br \/>25-100\u2103\u6c34\u6d74\u7bb1\u7528\u4e8e\u5e38\u89c4\u8bd5\u9a8c\u3002<br \/>5. \u70d8\u7bb1\uff1a\u4e3b\u7528\u4e8e\u70d8\u5e72\u5b9e\u9a8c\u5668\u76bf\uff0c\u6709\u4e9b\u9700\u8981\u6e29\u5ea6\u9ad8\u4e9b\uff0c\u6709\u4e9b\u9700\u8981\u6e29\u5ea6\u4f4e\u4e9b\u3002\u7528\u4e8eRNA\u65b9\u9762\u7684\u5b9e\u9a8c\u7528\u5177\uff0c\u9700\u8981\u5728250\u2103\u70e4\u7bb1\u4e2d\u70d8\u5e72\uff0c\u6709\u4e9b\u5851\u6599\u7528\u5177\u53ea\u80fd\u572842-45\u2103\u7684\u70e4\u7bb1\u4e2d\u8fdb\u884c\u70d8\u5e72\u3002<br \/>\uff08\u4e8c\uff09\u6c34\u7684\u51c0\u5316\u88c5\u7f6e\uff1a\u968f\u7740\u5206\u5b50\u751f\u7269\u5b66\u7684\u98de\u901f\u53d1\u5c55\uff0c\u8bb8\u591a\u5b9e\u9a8c\u5bf9\u6c34\u7eaf\u5ea6\u7684\u8981\u6c42\u8d8a\u6765\u8d8a\u9ad8\u3002<br \/>1. \u84b8\u998f\u6c34\u76bf\uff1a\u5355\u84b8\u6c34\u5e38\u96be\u4ee5\u6ee1\u8db3\u5b9e\u9a8c\u8981\u6c42\u3002\u53cc\u84b8\u6c34\u3001\u4e09\u84b8\u6c34\u914d\u6db2\uff0c\u8bb8\u591a\u5b9e\u9a8c\u8981\u53bb\u79bb\u5b50\u6c34\u3002<br \/>\u591a\u6b21\u84b8\u998f\u6c34\u53ef\u9664\u53bb\u6c34\u4e2d\u6325\u53d1\u6027\u6742\u8d28\uff0c\u4e0d\u80fd\u5b8c\u5168\u9664\u53bb\u6c34\u4e2d\u6eb6\u89e3\u7684\u6c14\u4f53\u6742\u8d28\uff08Mn2+\u3001Cu2+\u3001Zn2+\u3001Fe3+\u3001Mo(\u2165)\uff09\u3002<br \/>2. \u79bb\u5b50\u4ea4\u6362\u5668\uff1a\u53bb\u79bb\u5b50\u6c34\u2014\u7528\u79bb\u5b50\u4ea4\u6362\u6cd5\u5236\u53d6\u7684\u6c34\uff0c\u79f0\u53bb\u79bb\u5b50\u6c34\u3002<br \/>\u53bb\u79bb\u5b50\u6548\u679c\u597d\uff0c\u4f46\u4e0d\u80fd\u9664\u53bb\u6c34\u4e2d\u7684\u975e\u79bb\u5b50\u578b\u6742\u8d28\uff0c\u5176\u4e2d\u5e38\u542b\u6709\u5fae\u91cf\u7684\u6709\u673a\u7269\uff08\u6811\u8102\u7b49\uff09\u3002<br \/>1<br \/>3\uff0e\u8d85\u7eaf\u6c34\uff1a\u7528\u84b8\u998f\u6c34\u3001\u79bb\u5b50\u4ea4\u6362\u6c34\u3001\u53cd\u6e17\u900f\u7eaf\u6c34\u505a\u4e3a\u4f9b\u6c34\uff0c\u78c1\u94c1\u8026\u5408\u9f7f\u8f6e\u6cf5\u4f5c\u7528\u4f7f\u6c34\u5faa\u73af\u3002\u7528\u4e8ePCR\u3001PCR\u6c28\u57fa\u9178\u5206\u6790\u3001DNA\u6d4b\u5e8f\u3001\u9176\u53cd\u5e94\u3001\u7ec4\u7ec7\u548c\u7ec6\u80de\u57f9\u517b\u7b49\u3002<br \/>\uff08\u4e09\uff09\u83cc\u6d88\u6bd2\u8bbe\u5907\uff1a\u5206\u5b50\u751f\u7269\u5b66\u6240\u7528\u5927\u90e8\u5206\u8bd5\u5242\uff0c\u800c\u4e14\u5b9e\u9a8c\u7528\u5177\u90fd\u5e94\u4e25\u683c\u6d88\u6bd2\u706d\u83cc\u3002\u3002<br \/>1\uff0e\u84b8\u6c7d\u6d88\u6bd2\u9505\uff1a\u7528\u4e8e\u5c0f\u6279\u91cf\u7269\u54c1\u7684\u968f\u65f6\u6d88\u6bd2\u3002\u5927\u6279\u5b9e\u9a8c\u7269\u54c1\u3001\u8bd5\u5242\u3001\u57f9\u517b\u57fa\u53ef\u4f7f\u7528\u5927\u578b\u6d88\u6bd2\u4e14\u5b9a\u65f6\u8fdb\u884c\u6d88\u6bd2<br \/>2. \u7d2b\u5916\u7ebf\uff1a75%\u4e59\u9187 \u30010.1%SDS\uff08\u6d88\u6bd2\u5242\uff09<br \/>\u4e00\u4e9b\u8010\u9ad8\u538b\u3001\u9ad8\u6e29\u6d88\u6bd2\u4e14\u53ef\u7528\u7d2b\u5916\u7ebf\u7167\u5c04\u6216\u4e59\u9187\u548cSDS\u6d78\u6ce1\u3002<br \/>\u7d2b\u5916\u7ebf\u7167\u5c04\u4f7f\u7528\u65b9\u4fbf\uff0c\u4e14\u5f88\u65b9\u4fbf\uff0c\u4f46\u706d\u83cc\u6548\u679c\u4e0e\u8ddd\u79bb\u6709\u5173\uff0c\u4e14\u4ea7\u751f\u81ed\u6c27\u6c61\u67d3\u5b89\u5168\uff0c\u7528\u4e8e\u65e0\u83cc\u5ba4\uff0c\u8d85\u51c0\u53f0\u548c\u5851\u6599\u7528\u5177\u7684\u6d88\u6bd2\u3002<br \/>3. \u6ee4\u5668\u6ee4\u819c\uff1a\u4e0d\u8010\u9ad8\u6e29\u3001\u9ad8\u538b\u7684\u8bd5\u5242\u7528\u5176\u5904\u83cc\u3002<br \/>4. \u716e\u6cb8\u6d88\u6bd2\uff1a\u4e3b\u7528\u4e8e\u91d1\u5c5e\u5668\u68b0\u548c\u9488\u6025\u9700\u65f6\u91c7\u7528\u3002<br \/>\uff08\u56db\uff09 \u8ba1\u91cf\u7cfb\u7edf\uff1a<br \/>1. \u79f0\u91cf\u7cfb\u7edf\uff1a\uff08\u5404\u79cd\u5929\u5e73\uff09\u53f0\u79e4\u3001\u6258\u76d8\u5929\u5e73\u3001\u94ae\u529b\u6446\u52a8\u5929\u5e73\u3001<br \/>\u5149\u7535\u5206\u5b50\u5929\u5e73\u3001\u7cbe\u5bc6\u7535\u5b50\u5206\u6790\u5929\u5e73<br \/>2. \u6db2\u4f53\u4f53\u79ef\u7684\u5ea6\u91cf\uff1a\u7cbe\uff1a\u91cf\u7b52\u3001\u79fb\u6db2\u7ba1\u3001\u5fae\u91cf\u53d6\u6db2\u5668<br \/>\u7c97\uff1a\u523b\u5ea6\u8bd5\u7ba1\u3001\u70e7\u676f\u3001\u9525\u5f62\u74f6<br \/>3. pH\u503c\u6d4b\u91cf\uff1a<br \/>pH\u8ba1\uff1a\u6d4b\u5b9a\u6eb6\u6db2\u4e2dH+\u7684\u76f4\u63a5\u7535\u4f4d\u7684\u4eea\u5668\uff0c\u4e3b\u8981\u901a\u8fc7\u4e00\u5bf9\u7535\u6781\uff0c\u5728\u4e0d\u540c\u7684pH\u6eb6\u6db2\u4e2d\u4ea7\u751f\u4e0d\u540c\u7684\u7535\u52a8\u52bf\u7528pH\u503c\u8868\u793a\u51fa\u6765\u3002<br \/>pH\u8bd5\u7eb8\uff1a\u53ea\u9002\u7528\u4e8e\u57f9\u517b\u6db2\u3001\u915a\u9971\u548c\u6db2\u3001\u7f13\u51b2\u6db2\u6216\u5176\u5b83\u8bd5\u5242\u6eb6\u6db2\u7684pH\u503c\u7684\u7c97\u7565\u4f30\u8ba1\u3002\u800c\u5927\u90e8\u5206\u8bd5\u5242\u7684\u914d\u5236\u8981\u6c42\u4e25\u683c\u7684pH\u503c\uff0c\u9700\u7cbe\u786e\u5ea6\u9ad8\uff08\u5c0f\u6570\u70b9\u540e\u4e24\u4f4d\uff09\u7684pH\u8ba1\u3002<br \/>4. OD\u503c\u6d4b\u91cf\uff1a\u5149\u5bc6\u5ea6\u3001\u5206\u5149\u5149\u5ea6\u8ba1\u662f\u5229\u7528\u7269\u8d28\u5728\u53ef\u89c1\u5149\u548c\u7d2b\u5916\u7ebf\u533a\u57df\u4e2d\u7684\u5438\u6536\u5149\u8c31\u6765\u9274\u5b9a\u8be5\u7269\u8d28\u7684\u6027\u8d28\u53ca\u5176\u542b\u91cf\u7684\u4e00\u79cd\u4eea\u5668\u3002\u5b83\u662f\u7531\u5149\u6e90\u3001\u5355\u8272\u5668\u3001\u5438\u6536\u6c60\u3001\u63a5\u6536\u5668\u3001\u6d4b\u91cf\u4eea\u8868\u6216\u663e\u793a\u5c4f\u5e55\u6240\u7ec4\u6210\u3002OD\u503c\u662f\u8bb8\u591a\u6eb6\u6db2\u4e2d\u6eb6\u8d28\u5b9a\u91cf\u7684\u65b9\u4fbf\u6307\u6807\u4e4b\u4e00\uff0c\u901a\u8fc7\u6240\u4ea7\u751f\u7684\u5355\u8272\u5149\u800c\u6d4b\u5b9a\u67d0\u4e00\u6eb6\u6db2\u5bf9\u8be5\u5355\u8272\u5149\u7684\u5438\u6536\u503c\uff0c\u5229\u7528\u5b83\u53ef\u8fdb\u884c\u6838\u9178\u6eb6\u6db2\u5b9a\u91cf\u548c\u7eaf\u5ea6\u7684\u521d\u6b65\u5224\u65ad\u3002<br \/>\uff08\u4e94\uff09\u79bb\u5fc3\u673a\uff1a\u79bb\u5fc3\u6280\u672f\u662f\u7814\u7a76\u751f\u7269\u7684\u7ed3\u6784\u548c\u529f\u80fd\u4e2d\u4e0d\u53ef\u7f3a\u5c11\u7684\u4e00\u79cd\u7269\u7406\u6280\u672f\u624b\u6bb5\u3002\u56e0\u4e3a\u5404\u79cd\u7269\u8d28\u5728\u6c89\u6dc0\u7cfb\u6570\u3001\u6d6e\u529b\u3001\u548c\u8d28\u91cf\u7b49\u65b9\u9762\u6709\u5dee\u5f02\uff0c\u53ef\u5229\u7528\u5f3a\u5927\u7684\u79bb\u5fc3\u529b\u573a\uff0c\u4f7f\u5176\u5206\u79bb\u3001\u7eaf\u5316\u548c\u6d53\u7f29\u3002\u76ee\u524d\u6709\u5404\u79cd\u5404\u6837\u7684\u79bb\u5fc3\u673a\u3002\u53ef\u4f9b\u5c11\u4e8e0.05ml\u5230\u51e0\u5347\u7684\u6837\u54c1\u79bb\u5fc3\u4e4b\u7528\u3002\u79bb\u5fc3\u6280\u672f\u5e94\u7528\u5e7f\u6cdb\uff0c\u5305\u62ec\u6536\u96c6\u548c\u5206\u79bb\u7ec6\u80de\u3001\u7ec6\u80de\u5668\u548c\u751f\u7269\u5927\u5206\u5b50\u7b49\u3002\u636e\u5176\u8f6c\u901f\u7684\u4e0d\u540c\uff0c\u53ef\u5206\u4e3a\u4ee5\u4e0b\u51e0\u79cd\u7c7b\u578b\uff1a<br \/>1. \u666e\u901a\u79bb\u5fc3\u673a\uff1a\u6700\u5927\u8f6c\u901f6000 r\/m \uff0c\u6700\u5927\u79bb\u5fc3\u529b6000g<br \/>\u2460\u533b\u7528\u6216\u53f0\u5f0f\u79bb\u5fc3\u673a\uff1a\u662f\u79bb\u5fc3\u673a\u4e2d\u6700\u7b80\u5355\u800c\u5ec9\u4ef7\u7684\uff0c\u6700\u5e38\u7528\u4e8e\u6536\u96c6\u5feb\u901f\u6c89\u964d\u7cfb\u6570\u7684\u7269\u8d28\uff0c\u5982\u7ea2\u7ec6\u80de\u3001\u7c97\u5927\u7684\u6c89\u6dc0\u7269\u3001\u9175\u6bcd\u83cc\u548c\u7ec6\u83cc\u7b49\u3002<br \/>\u2461\u4f4e\u901f\u51b7\u51bb\u79bb\u5fc3\u673a\uff1a\u4e3b\u8981\u7528\u4e8e\u7ec6\u80de\u3001\u7ec6\u80de\u6838\u3001\u7ec6\u80de\u819c\u548c\u7ec6\u83cc\u7684\u6c89\u6dc0\u548c\u6536\u96c6\u7b49\u3002 2<br \/>2. \u9ad8\u901f\u79bb\u5fc3\u673a\uff1a\u6700\u5927\u8f6c\u901f25000 r\/m \uff0c\u6700\u5927\u79bb\u5fc3\u529b89000g<br \/>\u6709\u51b7\u51bb\u548c\u5e38\u6e29\u4e24\u79cd\uff0c\u591a\u7528\u4e8e\u5236\u5907\u548c\u624b\u6536\u96c6\u5fae\u751f\u7269\u3001\u7ec6\u80de\u788e\u7247\u3001\u7ec6\u80de\u3001\u5927\u7684\u7ec6\u80de\u5668\u3001\u786b\u9178\u94f5\u6c89\u6dc0\u7269\u4ee5\u53ca\u514d\u75ab\u6c89\u6dc0\u7269\u7b49\u3002<br \/>3. \u8d85\u901f\u79bb\u5fc3\u673a\uff1a\u6700\u5927\u8f6c\u901f9000 r\/m \uff0c\u6700\u5927\u79bb\u5fc3\u529b694000g\u3002<br \/>4. \u53f0\u5f0f\u8d85\u901f\u79bb\u5fc3\u673a\uff1a\u6700\u5927\u8f6c\u901f12000r\/m \uff0c\u6700\u5927\u79bb\u5fc3\u529b625000g\u3002<br \/>\uff08\u516d\uff09\u8d85\u51c0\u5de5\u4f5c\u53f0\uff1a\u5185\u6709\u7d2b\u5916\u706f\u3001\u7167\u660e\u706f\u3001\u8fd8\u5e94\u6709\u9152\u7cbe\u706f\u706b\u7130\u300175%\u4e59\u9187\u7b49\u706d\u83cc\u7684\u8bbe\u5907\uff0c\u662f\u4e00\u79cd\u63d0\u4f9b\u5c40\u90e8\u6d01\u51c0\u5ea6\u7684\u8bbe\u5907\u3002\u5176\u539f\u7406\u662f\u9f13\u98ce\u673a\u9a71\u52a8\u7a7a\u6c14\uff0c\u7ecf\u8fc7\u4f4e\u3001\u4e2d\u6548\u7684\u8fc7\u6ee4\u5668\u540e\uff0c\u901a\u8fc7\u5de5\u4f5c\u53f0\u9762\uff0c\u4f7f\u5b9e\u9a8c\u64cd\u4f5c\u533a\u57df\u6210\u4e3a\u65e0\u83cc\u7684\u73af\u5883\u3002\u8d85\u51c0\u53f0\u6309\u6c14\u6d41\u65b9\u5411\u7684\u4e0d\u540c\u5927\u81f4\u6709\u51e0\u79cd\u7c7b\u578b\uff1a<br \/>\u2460\u4fa7\u6d41\u5f0f\uff1a\u51c0\u5316\u540e\u6c14\u6d41\uff0c\u4ece\u5de6\u4fa7\u6216\u53f3\u4fa7\u901a\u8fc7\u5de5\u4f5c\u53f0\u9762\uff0c\u6d41\u5411\u5bf9\u4fa7\uff0c\u6216\u8005\u4ece\u4e0a\u5f80\u4e0b\u6216\u4ece\u4e0b\u5f80\u4e0a\u6d41\u5411\u5bf9\u4fa7\uff0c\u4ed6\u4eec\u90fd\u80fd\u5f62\u6210\u6c14\u6d41\u5c4f\u969c\u800c\u4fdd\u969c\u53f0\u9762\u65e0\u83cc\u3002<br \/>\u7f3a\u70b9\uff1a\u5728\u51c0\u5316\u6c14\u6d41\u548c\u5916\u8fb9\u6c14\u4f53\u4ea4\u754c\u5904\uff0c\u53ef\u56e0\u6c14\u4f53\u7684\u6d41\u5411\u800c\u51fa\u73b0\u8d1f\u538b\uff0c\u4f7f\u5c11\u91cf\u7684\u672a\u51c0\u5316\u6c14\u4f53\u6df7\u5165\uff0c\u800c\u9020\u6210\u6c61\u67d3\u3002<br \/>\u2461\u5916\u6d41\u5f0f\uff1a\u6c14\u6d41\u662f\u9762\u5411\u64cd\u4f5c\u4eba\u5458\u7684\u65b9\u5411\u6d41\u52a8\uff0c\u4ece\u800c\u4fdd\u8bc1\u5916\u9762\u6c14\u4f53\u4e0d\u80fd\u6df7\u5165\u3002<br \/>\u7f3a\u70b9\uff1a\u5728\u8fdb\u884c\u6709\u5bb3\u7269\u8d28\u5b9e\u9a8c\u65f6\uff0c\u5bf9\u64cd\u4f5c\u4eba\u5458\u4e0d\u5229\uff0c\u4f46\u53ef\u91c7\u7528\u6709\u673a\u73bb\u7483\u628a\u4e0a\u534a\u90e8\u5206\u906e\u6321\u8d77\u6765\uff0c\u4f7f\u6c14\u6d41\u5f80\u4e0b\u65b9\u6d41\u51fa\u3002\uff08\u4e03\uff09\u7535\u6cf3\u7cfb\u7edf\uff1a\u7535\u6cf3\u6280\u672f\u662f\u68c0\u6d4b\u3001\u9274\u5b9a\u5404\u79cd\u751f\u7269\u5927\u5206\u5b50\u7684\u7eaf\u5ea6\u3001\u542b\u91cf\u53ca\u63cf\u8ff0\u5b83\u4eec\u7684\u7279\u5f81\uff0c\u751a\u81f3\u8fd8\u662f\u5206\u79bb\u3001\u7eaf\u5316\u3001\u56de\u6536\u548c\u6d53\u7f29\u6837\u54c1\u7684\u5de5\u5177\u4e4b\u4e00\u3002\u6838\u9178\u548c\u86cb\u767d\u8d28\u7b49\u90fd\u5e26\u6709\u7535\u8377\uff0c\u5f53\u5b83\u4eec\u88ab\u7f6e\u4e8e\u7535\u573a\u4e2d\u65f6\uff0c\u80fd\u591f\u79fb\u52a8\u3002<br \/>\u7535\u6cf3\u88c5\u7f6e\u7531\u4e24\u90e8\u5206\u7ec4\u6210\uff1a\u7535\u6e90\u88c5\u7f6e\u548c\u7535\u6cf3\u69fd\u88c5\u7f6e\u3002<br \/>\u2460 \u7535\u6cf3\u88c5\u7f6e\uff1a\u7535\u6e90\u9700\u7ecf\u7a33\u6d41\u901a\u8fc7\u7a33\u538b\u5668\uff0c\u65e2\u80fd\u63d0\u4f9b\u7a33\u5b9a\u7684\u76f4\u6d41\u7535\uff0c\u53c8\u80fd\u8f93\u51fa\u7a33\u5b9a\u7684\u7535\u538b\u3002\u53ef\u7528\u4e8e\u4e09\u79cd\uff1a<br \/>\u5e38\u5ea6\u7a33\u538b\u7535\u6cf3\u4eea\uff1a\u8f93\u51fa\u7535\u538b0-500v 0-15mA<br \/>\u4e2d\u5ea6\u7a33\u538b\u7535\u6cf3\u4eea\uff1a\u8f93\u51fa\u7535\u538b400-1000v<br \/>\u9ad8\u5ea6\u7a33\u538b\u7535\u6cf3\u4eea\uff1a\u8f93\u51fa\u7535\u538b1000\u4ee5\u4e0a\u7684\u7535\u6e90\u88c5\u7f6e\u3002<br \/>\u2461 \u7535\u6cf3\u69fd\u88c5\u7f6e\uff1a\u5206\u4e24\u79cd<br \/>\u6c34\u5e73\u5f0f\u7535\u6cf3\u69fd\uff1a\u4e00\u822c\u5206\u4e3a\u5fae\u578b\u7535\u6cf3\u69fd\u548c\u5927\u53f7\u6c34\u5e73\u5f0f\u7535\u6cf3\u69fd<br \/>\u5782\u76f4\u5f0f\u7535\u6cf3\u69fd\uff1a\u5206\u5782\u76f4\u5e73\u677f\u7535\u6cf3\u69fd\u548c\u5706\u67f1\u5f62\u7535\u6cf3\u69fd\u88c5\u7f6e\u3002<br \/>\uff08\u516b\uff09PCR\u4eea\uff1aPolymerase Chain Reaction\u4eea\uff0c\u4e5f\u79f0DNA\u70ed\u5faa\u73af\u4eea\uff0c\u57fa\u56e0\u6269\u589e\uff0c\u5b83\u662f\u4f7f\u4e00\u5bf9\u5be1\u6838\u82f7\u9178\u5f15\u7269\u7ed3\u5408\u5230\u6b63\u8d1fDNA\u94fe\u4e0a\u7684\u9776\u5e8f\u5217\u4e24\u4fa7\uff0c\u4ece\u800c\u9176\u4fc3\u5408\u6210\u62f7\u8d1d\u6570\u767e\u4e07\u500d\u7684\u9776\u5e8f\u5217DNA\u7247\u6bb5\uff0c\u5b83\u7684\u6bcf\u4e00\u5faa\u73af\u5305\u62ec\u5728\u4e09\u79cd\u4e0d\u540c\u6e29\u5ea6\u8fdb\u884c\u7684DNA\u53d8\u6027\uff0c\u5f15\u7269\u590d\u6027\uff0cDNA\u805a\u5408\u9176\u50ac\u5316\u7684\u5ef6\u4f38\u53cd\u5e94\u4e09\u4e2a\u8fc7\u7a0b\u3002<br \/>\uff08\u4e5d\uff09\u51dd\u80f6\u6210\u50cf\u5206\u6790\u7cfb\u7edf:<br \/>\u5bf9\u7535\u6cf3\u540e\u542b\u6eb4\u4e59\u952d\uff08EB\uff09\u6838\u9178\u6837\u54c1\u7684\u89c2\u5bdf\u5206\u6790\u3002<br \/>\uff08\u5341\uff09\u5e72\u71e5\u8bbe\u5907\uff1a 3<br \/>1. \u771f\u7a7a\u52a0\u70ed\u5e72\u71e5\u7bb1\uff1a\u6838\u9178\u5728\u785d\u9178\u7ea4\u7ef4\u7d20\u819c\u548c\u5c3c\u9f99\u819c\u4e0a\u56fa\u5b9a\uff0c\u7528\u4e8e\u6742\u4ea4\u5b9e\u9a8c\u3002<br \/>2. \u7535\u6cf3\u51dd\u80f6\u5e72\u71e5\u7bb1\uff1a\u7535\u6cf3\u540e\u7684\u51dd\u80f6\u8fdb\u884c\u8131\u6c34\u5e72\u71e5\u7684\u4eea\u5668\uff0c\u4e00\u822c\u53ef\u5c06\u51dd\u80f6\u5e72\u71e5\u5230\u4e00\u4e9b\u73bb\u7483\u7eb8\u4e0a\uff0c\u5e72\u71e5\u540e\u7684\u51dd\u80f6\u6613\u4e8e\u4fdd\u5b58\u3002<br \/>3. \u6db2\u6c2e\u51b7\u51bb\u5e72\u71e5\uff1a\u9002\u7528\u4e8e\u6d3b\u6027\u86cb\u767d\u8d28\u6837\u54c1\u7684\u5e72\u71e5\u4e0e\u7ed3\u6676\u3002<br \/>4. \u771f\u7a7a\u6cf5\uff1a\u8bb8\u591a\u5b9e\u9a8c\u90fd\u9700\u8981\u62bd\u771f\u7a7a\u3002\u5982\uff1a\u4e59\u9187\u6c89\u6dc0\u540e\u6838\u9178\u6837\u54c1\u7684\u5e72\u71e5\uff0c\u7535\u6cf3\u51dd\u80f6\u7684\u5e72\u71e5\u7b49\u3002<br \/>\uff08\u5341\u4e00\uff09 \u5176\u4ed6<br \/>1. \u5fae\u6ce2\u7089\uff1a\u4fbf\u4e8e\u4e00\u4e9b\u6eb6\u6db2\u7684\u5feb\u901f\u52a0\u70ed\u548c\u5b9a\u6e29\u52a0\u70ed\uff0c\u7535\u6cf3\u743c\u8102\u7cd6\u51dd\u80f6\u914d\u5236\u3001\u6eb6\u5316\u7b49\u3002<br \/>2. \u5236\u51b0\u673a\uff1a\u7528\u4e8e\u5236\u9020\u5927\u591a\u6570\u6838\u9178\u3001\u86cb\u767d\u8d28\u7684\u5b9e\u9a8c\u64cd\u4f5c\u6240\u9700\u7684\u4f4e\u6e29\u73af\u5883\uff0c\u4ee5\u51cf\u5c11\u6838\u9178\u9176\u6216\u86cb\u767d\u8d28\u9176\u7684\u6c34\u89e3\u3002<br \/>3. \u5c42\u6790\u88c5\u7f6e\uff1a\uff08\u8272\u8c31\u5206\u79bb\uff09\u662f\u4e00\u79cd\u5206\u79bb\u591a\u7ec4\u5206\u6df7\u5408\u7269\u7684\u6709\u6548\u7269\u7406\u65b9\u6cd5\u3002<br \/>\u771f\u7a7a\u5370\u8bb0\u7cfb\u7edf\uff0cDNA\u5408\u6210\/\u6d4b\u5e8f\u4eea\uff1a\u8fd9\u4e9b\u90fd\u662f\u5bf9\u6838\u9178\u8fdb\u884c\u6df1\u5165\u7814\u7a76\u7684\u5fc5\u5907\u4eea\u5668\u3002<br \/>4. \u78c1\u529b\u6405\u62cc\u5668\uff1a\u591a\u89d2\u5ea6\u65cb\u8f6c\u6df7\u5300\u5668\u3001\u5feb\u901f\u632f\u8361\u6df7\u5408\u5668\uff1a\u7528\u4e8e\u6df7\u5408\u4eea\u5668\u3002<br \/>5. \u7ec4\u7ec7\u5300\u6d46\u5668\uff1a\u8d85\u58f0\u7ec4\u7ec7\u53ca\u7ec6\u80de\u7834\u788e\u5668\uff0c\u7528\u5176\u8fdb\u884c\u6837\u54c1\u7684\u5206\u79bb\u63d0\u7eaf\u5b9e\u9a8c\u3002<br \/>6. \u901a\u98ce\u6a71\uff1a\u5f88\u591a\u6eb6\u5242\u80fd\u9038\u51fa\u6bd2\u6c14\uff0c\u5fc5\u5907\u67dc \uff0c\u653e\u5c04\u6027\u8bd5\u9a8c\u8fd8\u8981\u6709\u6709\u673a\u73bb\u7483\u5c4f\u853d\u3002<br \/>7. \u73bb\u7483\u84b8\u998f\u5668\u3001\u7535\u70ed\u52a0\u5e3d\u3001\u53d8\u538b\u5668\uff1a\u7528\u4e8e\u915a\u7b49\u6709\u673a\u6eb6\u5242\u7684\u84b8\u998f\u3002<br \/>8. Tip\u5934\u3001Eppendorf\u7ba1\uff1a<br \/>\u5fae\u7ba1\u79fb\u6db2\u5668tip\u5934\uff08\u5438\u6db2\u5c16\uff09\u3001Eppendorf\u7ba1\uff08\u5fae\u91cf\u79bb\u5fc3\u7ba1\uff09\u53ef\u6d17\u6da4\uff0c\u7845\u5316\u6d88\u6bd2\u540e\u53cd\u590d\u4f7f\u7528\u3002\u5bf9\u4e00\u4e9b\u8981\u6c42\u4e25\u683c\u7684\u5b9e\u9a8c\uff0c\u5982RNA\u7684\u63d0\u53d6\u3001\u4fdd\u5b58\u7b49\u64cd\u4f5c\uff0c\u5e94\u4f7f\u7528\u65b0\u7684\u6d88\u6bd2tip\u5934\u4e0eEppendorf\u7ba1\u3002\u53e6\u5916\u8fd8\u5e94\u5907\u6709\u5e38\u7528\u89c4\u683c\u7684\u79bb\u5fc3\u7ba1\uff081000ml\u3001500 ml\u3001250 ml\u300150 ml\u30017 ml\u7b49\uff09\u53ca96\u5b54\u300124\u5b54\u300112\u5b54\u30016\u5b54\u7684\u7ec6\u80de\u57f9\u517b\u5851\u6599\u5e73\u677f\u7b49\u3002<br \/>9. \u5c0f\u578b\u8bbe\u5907\u3001\u7528\u5177\uff1a<br \/>\u5b9a\u65f6\u5668\u3001\u6ee4\u819c\u3001\u4fdd\u9c9c\u819c\u3001\u9632\u62a4\u773c\u955c\u9e2d\u5634\u954a\u3001\u5e38\u89c4\u7684\u73bb\u7483\u6216\u5851\u6599\u5668\u76bf\uff08\u5305\u62ec\u5e73\u76bf\u3001\u8bd5\u7ba1\u3001\u70e7\u676f\u3001\u91cf\u74f6\u3001\u8bd5\u5242\u5206\u6db2\u6f0f\u6597\u3001\u907f\u5149\u4fdd\u5b58\u7684\u8bd5\u5242\u5e94\u4f7f\u7528\u68d5\u8272\u8bd5\u5242\u74f6\uff0c\u5982\u9971\u548c\u915a\u3001\u5def\u57fa\u4e59\u9187\u7b49\uff09\u3001\u8bb0\u53f7\u7b14\u3001\u5404\u79cd\u624b\u5957PE\u3001\u4e73\u80f6\u3001\u5bb6\u7528\u3001\u9632\u9178\u7684\u7b49\uff09<br \/>\u4e8c\u3001\u672c\u671f\u5b9e\u9a8c\u6240\u7528\u4e3b\u8981\u4eea\u5668\u3001 \u8bbe\u5907\u7684\u529f\u80fd\u53ca\u64cd\u4f5c<br \/>1. \u915a\u7684\u91cd\u84b8\u998f\u88c5\u7f6e\uff1a\u7a7a\u6c14\u51b7\u51dd\u5f0f\u73bb\u7483\u84b8\u998f\u5668\u3002<br \/>\u4e00\u822c\u915a\u662f\u65e0\u8272\u900f\u660e\u7684\u7ed3\u6676\uff0c\u5982\u5448\u7c89\u8272\u6216\u9ec4\u8272\uff0c\u8868\u660e\u5176\u4e2d\u542b\u6709\u915a\u7684\u6c27\u5316\u4ea7\u7269\uff0c\u5982\u918c\u3001\u4e8c\u9178\u7b49\uff0c\u53d8\u8272\u7684\u915a\u4e0d\u80fd\u7528\u4e8e\u5b9e\u9a8c\uff0c\u56e0\u5176\u4e2d\u7684\u915a\u6c27\u5316\u7269\u53ef\u7834\u574f\u6838\u9178\u7684\u78f7\u9178\u4e8c\u916f\u952e\uff0c\u5e76\u5f15\u8d77DNA\u94fe\u7684\u4ea4\u8054\u3002\u4f7f\u7528\u524d\u5fc5\u987b\u5728160\u2103-180\u2103\u91cd\u84b8\u998f\u4ee5\u53bb\u9664\u80fd\u5f15\u8d77RNA\u548cDNA\u65ad\u88c2\u548c\u4ea4\u8054\u7684\u6c61\u67d3\u6742\u8d28\uff0c\u4ece\u800c\u5f97\u5230\u7eaf\u51c0\u7684\u915a\u3002<br \/>2. \u78c1\u529b\u6405\u62cc\u5668\uff1a81-2\u578b\u6052\u6e29\u78c1\u529b\u6405\u62cc\u5668<br \/>\u5c06\u5bfc\u7535\u6e29\u5ea6\u8868\u7528\u5bfc\u7ebf\u4e0e\u4e8c\u82af\u63d2\u5934\u8fde\u63a5\uff0c\u63d2\u5165\u540e\u9762\u7684\u6e29\u5ea6\u8868\u5b54\u5185\uff0c\u5f53\u6e29\u5ea6\u4e0a\u5347\u5230\u9884\u5148\u8c03\u6574\u597d\u7684\u5ea6\u6570\u65f6\u5373\u81ea\u52a8\u505c\u6b62\u52a0\u70ed\uff08\u63a7\u6e29\u6405\u62cc\uff09\u3002\u4e3b\u8981\u7528\u4e8e\u7269\u8d28\u7684\u5feb\u901f\u6405\u62cc\u3001\u6eb6\u89e3\u548c\u6df7\u5300\u3002\u5982\u914d\u5236\u8bd5\u5242\uff0c\u5236\u5907\u915a\u7684\u6c34\u9971\u548c\u6db2\u7b49\u3002 4<br \/>3. \u84b8\u998f\u6c34\u5668\uff1a1810B\u81ea\u52a8\u53cc\u91cd\u7eaf\u6c34\u84b8\u998f\u5668\uff0c\u77f3\u82f1\u7ba1\u52a0\u70ed\u5f0f\uff0c\u672c\u5668\u7684\u4e00\u6b21\u53ca\u4e8c\u6b21\u84b8\u998f\u5747\u6709\u4fdd\u62a4\u88c5\u7f6e\uff0c\u4f7f\u7528\u5b89\u5168\u53ef\u9760\uff0c\u70ed\u7ee7\u7535\u5668\u5f53\u51b7\u5374\u6c34\u7a81\u7136\u4e2d\u65ad\u6216\u7f3a\u6c34\u7684\u60c5\u51b5\u4e0b\uff0c\u5373\u81ea\u52a8\u5207\u65ad\u7535\u6e90\u3002\u5e72\u7c27\u7ee7\u7535\u5668\u63a7\u5236\u4e8c\u6b21\u84b8\u998f\u74f6\u5185\u7684\u6c34\u4f4d\uff0c\u5176\u9ad8\u5ea6\u5e94\u4ee5\u5728\u6c34\u4f4d\u964d\u4f4e\u65f6\u80fd\u81ea\u52a8\u5207\u65ad\u7535\u6e90\u4e3a\u51c6\uff0c\u4ece\u800c\u8fbe\u5230\u81ea\u52a8\u63a7\u5236\u6c34\u4f4d\u7684\u76ee\u7684\u3002<br \/>\u8be5\u4eea\u5668\u4e3b\u8981\u7528\u4e8e\u5236\u5907\u53cc\u84b8\u6c34\u548c\u4e09\u84b8\u6c34\u3002<br \/>4. \u79bb\u5fc3\u673a\uff1aTGL-16G\u53f0\u5f0f\u9ad8\u901f\u79bb\u5fc3\u673a\uff0c\u6700\u5927\u8f6c\u901f20000rpm\uff0c\u4e3b\u8981\u7528\u4e8e\u6838\u9178\u63d0\u53d6\u65f6\u86cb\u767d\u8d28\u7684\u6c89\u6dc0\u548c\u6709\u673a\u76f8\u4e0e\u6c34\u76f8\u7684\u5206\u5c42\uff0cPCR\u68c0\u6d4b\u7684\u77ac\u65f6\u79bb\u5fc3\u3002<br \/>5. \u5fae\u91cf\u53d6\u6db2\u5668\uff1a\u4e3b\u8981\u7528\u4e8e\u7cbe\u786e\u91cf\u53d6\u5fae\u91cf\u6db2\u4f53\u4f53\u79ef\u548c\u6838\u9178\u6c34\u76f8\u7684\u8f6c\u79fb\u3002<br \/>6. \u5300\u6d46\u5668\uff1a5ml\u300110ml\u73bb\u7483\u5300\u6d46\u5668\u3002<br \/>\u4e3b\u7528\u4e8e\u7ec4\u7ec7\u7ec6\u80de\u7684\u7834\u788e\u3002<br \/>7. \u51dd\u80f6\u6210\u50cf\u5206\u6790\u7cfb\u7edf<br \/>\u4e3b\u7528\u4e8e\u6838\u9178\u6837\u54c1\u743c\u8102\u7cd6\u51dd\u80f6\u548cPCR\u7535\u6cf3\u7ed3\u679c\u7684\u7d2b\u5916\u89c2\u5bdf\u548c\u7167\u50cf\u3002<br \/>8. PCR\u4eea\uff1aPTC-200\u57fa\u56e0\u6269\u589e\u4eea<br \/>\u4f53\u5916\u6269\u589e\u6838\u9178\u7684\u4eea\u5668<br \/>9. \u84b8\u6c7d\u6d88\u6bd2\u9505\uff1a\u7528\u4e8e\u5b9e\u9a8c\u7528\u8bd5\u5242\u3001\u5668\u76bf\u3001\u7528\u5177\u7b49\u7684\u706d\u83cc\u6e05\u6bd2<br \/>\u6709\u7535\u52a0\u70ed\u5f0f\u3001\u7535\u7089\u52a0\u70ed\u5f0f\u4e24\u79cd\u3002<br \/>10. \u8d85\u51c0\u53f0\uff1aJJT-1300\u578b\u6d01\u51c0\u5de5\u4f5c\u53f0\uff0c\u4fa7\u6d41\u5f0f\u3002<br \/>\u539f\u7406\uff1a\u5438\u8fdb\u6c14\u7ecf\u8fc7\u521d\u3001\u4e2d\u7ea7\u8fc7\u6ee4\u76bf\uff08\u65e0\u7eba\u5e03\u6ee4\u8fc7\uff09\u540e\uff0c\u518d\u901a\u8fc7\u98ce\u673a\u7ecf\u9ad8\u7ea7\u8fc7\u6ee4\u5668\uff08\u8d85\u7ec6\u73bb\u7483\u7ea4\u7ef4\u6ee4\u7eb8\u5236\u6210\uff09\u800c\u5f62\u6210\u6d01\u51c0\u7a7a\u6c14\u3002\u4e3b\u8981\u662f\u5728\u5c40\u90e8\u7a7a\u6c14\u9020\u6210\u6d01\u51c0\u7a7a\u6c14\u73af\u5883\uff0c\u4ee5\u4f7f\u5de5\u4f5c\u533a\u57df\u4e2d\u7684\u60ac\u6d6e\u7c92\u5b50\u53ca\u5fae\u751f\u7269\u63a7\u5236\u5728\u6700\u4f4e\u9650\u5ea6\u5185\u3002<br \/>\u672c\u53f0\u64cd\u4f5c\u538b\u4e3a\u5782\u76f4\u5c42\u6d41\u538b\uff0c\u56e0\u6b64\u51fa\u98ce\u9762\u4e0e\u64cd\u4f5c\u4f4d\u7f6e\u4e0d\u5e94\u653e\u7f6e\u591a\u4f59\u7684\u7269\u54c1\uff0c\u4ee5\u514d\u59a8\u788d\u6c14\u6d41\u7684\u6b63\u5e38\u6d41\u52a8\uff0c\u5f71\u54cd\u6d01\u51c0\u5ea6\uff0c\u53f0\u9762\u677f\u4e0a\u7684\u5b54\u4f5c\u4e3a\u901a\u98ce\u5b54\uff0c\u4f7f\u7528\u65f6\u907f\u514d\u6709\u6db2\u4f53\u6216\u5176\u4ed6\u7269\u54c1\u6f0f\u5165\u7a7a\u4e2d\uff0c\u4ee5\u514d\u5f71\u54cd\u5185\u90e8\u6784\u4ef6\u3002\u98ce\u901f\u53ef\u8c03\u3002<br \/>\u4f7f\u7528\uff1a75%\u4e59\u9187\u6d88\u6bd22-3\u6b21\uff0c\u6446\u653e\u597d\u5b9e\u9a8c\u7528\u54c1\u3002\u63d2\u4e0a\u7535\u6e90\uff0c\u7d2b\u5916\u6d88\u6bd230\u5206\u949f\uff0c\u542f\u52a8\u98ce\u673a5m\u540e\u5173\u706f\uff0c\u5173\u706f\u540e10\u5206\u949f\u6253\u5f00\u7167\u660e\u706f\uff0c\u5373\u53ef\u4f7f\u7528\u3002<br \/>11. \u7535\u6cf3\u7cfb\u7edf\uff1a<br \/>\u2460\u7535\u6cf3\u4eea\uff1aHV-3000\u578b\u9ad8\u5ea6\u4e09\u6052\u591a\u7528\u7535\u6cf3\u4eea\uff08\u6052\u538b\u3001\u6052\u6d41\u3001\u6052\u529f\u7387\uff09<br \/>\u2461\u7535\u6cf3\u69fd\uff1a\u6c34\u5e73\u5f0f\u4e24\u79cd\u3002<br \/>12\uff0e\u5fae\u6ce2\u7089\uff1a\u4e3b\u8981\u7528\u4e8e\u743c\u8102\u7cd6\u7684\u5feb\u901f\u6eb6\u89e3\u3002<\/p>\n<p><p style=\"color: #444242;width: 100%;font-size: 60%;background: #F1F1F1\">\u4ee5\u4e0a\u6587\u7ae0\u662f\u5bf9<span class='wp_keywordlink'><a href=\"https:\/\/www.qipaizn.cn\/cljbpyg.html\" title=\"\u78c1\u529b\u6405\u62cc\u914d\u6db2\u7f50\" 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